Human LAB7-2 (B-Lymphocyte Activation Antigen B7-2) ELISA Kit | ELK1606

Human LAB7-2 (B-Lymphocyte Activation Antigen B7-2) ELISA Kit | ELK1606 | Gentaur UK, US & Europe Distribution

Alternative Names: CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2, B7-2 Antigen; CTLA-4 counter-receptor B7.2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.274 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Activation Antigen B7-2 (LAB7-2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphocyte Activation Antigen B7-2 (LAB7-2) in the samples is then determined by comparing the OD of the samples to the standard curve.