Description
Human LAB7-2 (B-Lymphocyte Activation Antigen B7-2) ELISA Kit | ELK1606 | Gentaur UK, US & Europe Distribution
Alternative Names: CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2, B7-2 Antigen; CTLA-4 counter-receptor B7.2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.274 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Lymphocyte Activation Antigen B7-2 (LAB7-2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Activation Antigen B7-2 (LAB7-2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphocyte Activation Antigen B7-2 (LAB7-2) in the samples is then determined by comparing the OD of the samples to the standard curve.