Human Lactase-phlorizin hydrolase (LCT) ELISA Kit | KTE61844

Human Lactase-phlorizin hydrolase (LCT) ELISA Kit | KTE61844 | Gentaur UK, US & Europe Distribution

Application: This Human Lactase-phlorizin hydrolase (LCT) ELISA Kit employs a two-site sandwich ELISA to quantitate LCT in samples. An antibody specific for LCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Lactase-phlorizin hydrolase microplate
• Human Lactase-phlorizin hydrolase standard
• Human Lactase-phlorizin hydrolase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Lactase-phlorizin hydrolase (LCT) ELISA Kit has high sensitivity and excellent specificity for detection of Human LCT. No significant cross-reactivity or interference between Human LCT and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: v-CRK avian sarcoma virus CT10-homolog-like contains one SH2 domain and two SH3 domains. CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase and plays a role in fibroblast transformation by BCR-ABL. In addition, CRKL has oncogenic potential.CrkL together with Crk participates in the Reelin signaling cascade downstream of DAB1.CRKL becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found CRKL to be a substrate for the BCR-ABL tyrosine kinase (ABL), leading them to conclude that CRKL is a tyrosine kinase and an oncogene.

Alternative Names: LCT; LAC; LPH; LPH1; lactase-glycosylceramidase; lactase-phlorizin hydrolase; lactase-phlorizin hydrolase-1

Search name: LCT; LAC; LPH; LPH1; lactase-glycosylceramidase; lactase-phlorizin hydrolase; lactase-phlorizin hydrolase-1

Tag: LCT