Description
Human LASS2 (Longevity Assurance Homolog 2) ELISA Kit | ELK7214 | Gentaur UK, US & Europe Distribution
Alternative Names: SP260; L3; TMSG1; CerS2; Longevity Assurance (LAG1) Homolog 2; Ceramide Synthase 2; LAG1 Longevity Assurance Homolog 2; Tumor Metastasis Suppressor Gene 1 Protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.058 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Longevity Assurance Homolog 2 (LASS2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Longevity Assurance Homolog 2 (LASS2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Longevity Assurance Homolog 2 (LASS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Longevity Assurance Homolog 2 (LASS2) in the samples is then determined by comparing the OD of the samples to the standard curve.