Human Late cornified envelope-like proline-rich protein 1 (LELP1) ELISA Kit | KTE61833

Human Late cornified envelope-like proline-rich protein 1 (LELP1) ELISA Kit | KTE61833 | Gentaur UK, US & Europe Distribution

Application: This Human Late cornified envelope-like proline-rich protein 1 (LELP1) ELISA Kit employs a two-site sandwich ELISA to quantitate LELP1 in samples. An antibody specific for LELP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLELP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LELP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LELP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Late cornified envelope-like proline-rich protein 1 microplate
• Human Late cornified envelope-like proline-rich protein 1 standard
• Human Late cornified envelope-like proline-rich protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Late cornified envelope-like proline-rich protein 1 (LELP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LELP1. No significant cross-reactivity or interference between Human LELP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: By searching for genes in a region of chromosome 1q21 associated with atopic dermatitis (ATOD2), Sharma et al. (2007) identified LELP1. By genomic sequence analysis, Sharma et al. (2007) mapped the LELP1 gene to chromosome 1q21. Interestingly, locus 5 is 6.2 kb upstream of a late cornified envelope-like proline-rich 1 (LELP1) gene which encodes a novel small proline rich protein. a significant association of rs7534334 (tagged SNP from LELP1) SNP with log10 serum IgE levels in the patient group (P=0.0029) . a chromosomal region in close proximity to a novel gene and highlight the need for intense research on LELP1 and other genes close by with respect to atopic disorders.

Alternative Names: LELP1; Novel small proline rich protein

Search name: LELP1; Novel small proline rich protein

Tag: LELP1