Human Late cornified envelope protein 3B (LCE3B) ELISA Kit | KTE61848

Human Late cornified envelope protein 3B (LCE3B) ELISA Kit | KTE61848 | Gentaur UK, US & Europe Distribution

Application: This Human Late cornified envelope protein 3B (LCE3B) ELISA Kit employs a two-site sandwich ELISA to quantitate LCE3B in samples. An antibody specific for LCE3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCE3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCE3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCE3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Late cornified envelope protein 3B microplate
• Human Late cornified envelope protein 3B standard
• Human Late cornified envelope protein 3B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Late cornified envelope protein 3B (LCE3B) ELISA Kit has high sensitivity and excellent specificity for detection of Human LCE3B. No significant cross-reactivity or interference between Human LCE3B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: By database analysis to identify human orthologs of mouse genes encoding late envelope proteins (LEPs), Marshall et al. (2001) identified LCE3B, which they called LEP14. RT-PCR detected strong LEP14 expression in human esophagus, but not in skin or heart.Using real-time PCR, Jackson et al. (2005) detected little to no LCE3B expression in human skin or internal epithelia. Jackson et al. (2005) determined that the LCE3B gene contains 2 exons. Exon 1 is noncoding.By genomic sequence analysis, Marshall et al. (2001) mapped the LCE3B gene within the LCE gene cluster on chromosome 1q21. Jackson et al. (2005) stated that the mouse Lce3b gene maps to a syntenic LCE gene cluster on chromosome 3F1.

Alternative Names: LCE3B; LEP14; late envelope protein 14

Search name: LCE3B; LEP14; late envelope protein 14

Tag: LCE3B