Human LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1) ELISA Kit | KTE61828

Human LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1) ELISA Kit | KTE61828 | Gentaur UK, US & Europe Distribution

Application: This Human LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1) ELISA Kit employs a two-site sandwich ELISA to quantitate LETM1 in samples. An antibody specific for LETM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLETM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LETM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LETM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human LETM1 and EF-hand domain-containing protein 1, mitochondrial microplate
• Human LETM1 and EF-hand domain-containing protein 1, mitochondrial standard
• Human LETM1 and EF-hand domain-containing protein 1, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human LETM1 and EF-hand domain-containing protein 1, mitochondrial (LETM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LETM1. No significant cross-reactivity or interference between Human LETM1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LETMD1 is involved in tumorigenesis and may function as a negative regulator of the TP53/p53. It is found in kidney, liver, skeletal muscle, heart and brain and is over-expressed in various tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon and uterine cervix. There are six isoforms.HCCR-2 that was overexpressed in various human tumors including leukemia, lymphoma, and carcinomas of the breast, kidney, ovary, stomach, colon, and uterine cervix. Ectopic expression of HCCR-2 resulted in direct tumorigenic conversions of NIH/3T3 and Rat1 fibroblasts. HCCR-2 probably represents a new oncogene that is related to tumorigenesis, functioning as a negative regulator of the p53 tumor suppressor.

Alternative Names: LETM1; LETM1 and EF-hand domain-containing protein 1; mitochondrial

Search name: LETM1; LETM1 and EF-hand domain-containing protein 1; mitochondrial

Tag: LETM1