Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit | AE20038HU

Human Leukocyte elastase inhibitor (SERPINB1) ELISA Kit | AE20038HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SERPINB1

Alternative Name: EI; ELANH2; LEI; M/NEI; MNEI; PI2; anti-elastase|protease inhibitor 2 (anti-elastase) ; monocyte/neutrophil derived|serine (or cysteine) proteinase inhibitor; clade B (ovalbumin) ; member 1

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.119 ng/mL

Intra-Assay: ≤4.3%

Inter-Assay: ≤6.5%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SERPINB1 in samples. An antibody specific for SERPINB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Monocyte/neutrophil elastase inhibitor (EI) is a protein of approximately 42, 000 Mr with serpin-like functional properties. Remold-O'Donnell et al. (1992) cloned EI cDNA and identified 3 EI mRNA species of 1.5, 1.9, and 2.6 kb in monocyte-like cells and no hybridizing mRNA in lymphoblastoid cells lacking detectable EI enzymatic activity. The cDNA open reading frame encoded a 379-amino acid protein. Its sequence established EI as a member of the serpin superfamily. Sequence alignment indicated that the reactive center P1 residue is cys-344, consistent with abrogation of elastase inhibitory activity by iodoacetamide and making EI a naturally occurring cys-serpin. Other elastase inhibitors include alpha-1-antitrypsin (PI) and secretory leukocyte protease inhibitor (SLPI) .

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .