Human LIM domain-binding protein 1 (LDB1) ELISA Kit | KTE61843

Human LIM domain-binding protein 1 (LDB1) ELISA Kit | KTE61843 | Gentaur UK, US & Europe Distribution

Application: This Human LIM domain-binding protein 1 (LDB1) ELISA Kit employs a two-site sandwich ELISA to quantitate LDB1 in samples. An antibody specific for LDB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human LIM domain-binding protein 1 microplate
• Human LIM domain-binding protein 1 standard
• Human LIM domain-binding protein 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human LIM domain-binding protein 1 (LDB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LDB1. No significant cross-reactivity or interference between Human LDB1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CLIM1 and CLIM2 have a very high level of identity to each other and to the homologs in other species.CLIM2 encodes a 375-amino acid protein.human LMO2 interacted with human BEX2. Electrophoretic mobility shift assays showed that BEX2 and LMO2 were part of a DNA-binding complex that recognized the E-box element. Other components of this complex included NSCL2 (NHLH2) and LDB1. LDB1 associated with a complex containing LMO4, CTIP, and BRCA1 in transfected human embryonic kidney cells.LDB1 was consistently enriched in the superior temporal gyrus posterior temporal cortex, and perisylvian cortex. LDB1 may be involved in asymmetric patterning of the developing human cortex.

Alternative Names: LDB1; CLIM2; NLI; LIM domain-binding factor-1; carboxy terminal LIM domain protein 2

Search name: LDB1; CLIM2; NLI; LIM domain-binding factor-1; carboxy terminal LIM domain protein 2

Tag: LDB1