Human LIM domain transcription factor LMO4 (LMO4) ELISA Kit | KTE62376

Human LIM domain transcription factor LMO4 (LMO4) ELISA Kit | KTE62376 | Gentaur UK, US & Europe Distribution

Application: This Human LIM domain transcription factor LMO4 (LMO4) ELISA Kit employs a two-site sandwich ELISA to quantitate LMO4 in samples. An antibody specific for LMO4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLMO4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LMO4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LMO4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human LIM domain transcription factor LMO4 microplate
• Human LIM domain transcription factor LMO4 standard
• Human LIM domain transcription factor LMO4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human LIM domain transcription factor LMO4 (LMO4) ELISA Kit has high sensitivity and excellent specificity for detection of Human LMO4. No significant cross-reactivity or interference between Human LMO4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LMO4 was expressed predominantly in the lobuloalveoli of the mammary gland during pregnancy. Consistent with a role in proliferation, forced expression of this gene inhibited differentiation of mammary epithelial cells. Overexpression of LMO4 mRNA was observed in 5 of 10 human breast cancer cell lines. Moreover, in situ hybridization analysis of 177 primary invasive breast carcinomas revealed overexpression of LMO4 in 56% of specimens. Immunohistochemistry confirmed overexpression in a high percentage (62%) of tumors. These studies implied a role for LMO4 in maintaining proliferation of mammary epithelium and suggested that deregulation of this gene may contribute to breast tumorigenesis.

Alternative Names: LMO4; OTTHUMP00000011906

Search name: LMO4; OTTHUMP00000011906

Tag: LMO4