Description
Human LMP2 (Large Multifunctional Peptidase 2) ELISA Kit | ELK2350 | Gentaur UK, US & Europe Distribution
Alternative Names: PSMB9; Proteasome (Prosome, Macropain) Subunit Beta Type 9; Macropain chain 7; Multicatalytic endopeptidase complex chain 7; Really interesting new gene 12 protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Large Multifunctional Peptidase 2 (LMP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Large Multifunctional Peptidase 2 (LMP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Large Multifunctional Peptidase 2 (LMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Large Multifunctional Peptidase 2 (LMP2) in the samples is then determined by comparing the OD of the samples to the standard curve.