Description
Human Lpa (Lipoprotein, a) ELISA Kit | ELK1564 | Gentaur UK, US & Europe Distribution
Alternative Names: Lp (a) ; AK38; APOA; LP; Sinking Pre-β-Lipoprotein
Species: Human
Assay Type: Sandwich
Sensitivity: 1.48 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Metabolic pathway;Cardiovascular biology;Hepatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipoprotein, a (Lpa) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lipoprotein, a (Lpa) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipoprotein, a (Lpa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipoprotein, a (Lpa) in the samples is then determined by comparing the OD of the samples to the standard curve.