Description
Human LRH1 (Liver Receptor Homolog 1) ELISA Kit | ELK4438 | Gentaur UK, US & Europe Distribution
Alternative Names: NR5A2; B1F; B1F2; CPF; FTF; FTZ-F1; LRH-1; hB1F; hB1F-2; Hepatocytic transcription factor; Alpha-1-fetoprotein transcription factor; Nuclear Receptor Subfamily 5, Group A, Member 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.112 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver Receptor Homolog 1 (LRH1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Liver Receptor Homolog 1 (LRH1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver Receptor Homolog 1 (LRH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Liver Receptor Homolog 1 (LRH1) in the samples is then determined by comparing the OD of the samples to the standard curve.