Human LTD4 (Leukotriene D4) ELISA Kit | ELK9178

Human LTD4 (Leukotriene D4) ELISA Kit | ELK9178 | Gentaur UK, US & Europe Distribution

Alternative Names: LTD4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.082 ng/mL

Standard: 20 ng/mL

Detection Range: 0.31-20 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Biochemicals

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukotriene D4 (LTD4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leukotriene D4 (LTD4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukotriene D4 (LTD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leukotriene D4 (LTD4) in the samples is then determined by comparing the OD of the samples to the standard curve.