Human Lymphocyte antigen 96 (LY96) ELISA Kit | KTE61765

Human Lymphocyte antigen 96 (LY96) ELISA Kit | KTE61765 | Gentaur UK, US & Europe Distribution

Application: This Human Lymphocyte antigen 96 (LY96) ELISA Kit employs a two-site sandwich ELISA to quantitate LY96 in samples. An antibody specific for LY96 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLY96 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY96 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY96 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Lymphocyte antigen 96 microplate
• Human Lymphocyte antigen 96 standard
• Human Lymphocyte antigen 96 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Lymphocyte antigen 96 (LY96) ELISA Kit has high sensitivity and excellent specificity for detection of Human LY96. No significant cross-reactivity or interference between Human LY96 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LY96 is involved in binding lipopolysaccharide with TLR 4. It is also known as "MD2". The MD-2 protein appears to associate with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. MD2 did not interact with LY64, but it did appear on cells expressing Toll-like receptor-4 (TLR4) . Toll-like receptors, which contain extracellular LRRs and have intracellular regions homologous to the intracellular signaling domain of the interleukin-1 receptor (IL1R), activate nuclear factor kappa-B (NFKB) . TLR4 is involved in the response to lipopolysaccharide (LPS) from gram-negative bacteria. Functional analysis showed that expression of MD2 enhanced TLR4-dependent activation of NFKB.

Alternative Names: LY96; MD-2; MD2; MD-2 protein; myeloid differentiation protein-2

Search name: LY96; MD-2; MD2; MD-2 protein; myeloid differentiation protein-2

Tag: LY96