Human Lysophosphatidic acid (LPA) ELISA Kit | AE62830HU

Human Lysophosphatidic acid (LPA) ELISA Kit | AE62830HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: LPA

Alternative Name: N/A

Application: ELISA

Range: 3.12-200 ng/mL

Sensitivity: 1.48 ng/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤9.3%

Recovery: 1, 04

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate LPA in samples. An antibody specific for LPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Lipoprotein (a) consists of an LDL-like particle and the specific apolipoprotein (a), which is covalently bound to the apoB of the LDL like particle. Lp (a) plasma concentrations are highly heritable and mainly controlled by the apolipoprotein (a) gene located on chromosome 6q26-27. Apo (a) proteins vary in size due to a size polymorphism, which is caused by a variable number of so called kringle IV repeats in the LPA gene. This size variation at the gene level is expressed on the protein level as well, resulting in apo (a) proteins with 10 to > 50 kringle IV repeats . These variable apo (a) sizes are known as "apo (a) isoforms". There is a general inverse correlation between the size of the apo (a) isoform and the Lp (a) plasma concentration which is caused by a variable rate of degradation before the apo (a) protein has matured for Lp (a) assembly.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .