Human ME2 (Malic Enzyme 2, NADP+ Dependent, Mitochondrial) ELISA Kit | ELK3646

Human ME2 (Malic Enzyme 2, NADP+ Dependent, Mitochondrial) ELISA Kit | ELK3646 | Gentaur UK, US & Europe Distribution

Alternative Names: Malate dehydrogenase; Malic enzyme 2; Malic enzyme 2 mitochondrial; Malic enzyme 2 NAD (+) dependent mitochondrial; Malic enzyme mitochondrial; Malic enzyme NAD (+) dependent mitochondrial; MAOM_HUMAN; ME 2; ME2; mitochondrial; NAD dependent malic enzyme mitochondrial; NAD ME; NAD-dependent malic enzyme; NAD-ME; ODS1; Pyruvic malic carboxylase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Malic Enzyme 2, NADP+ Dependent, Mitochondrial (ME2) in the samples is then determined by comparing the OD of the samples to the standard curve.