Description
Human MFI2 (Melanotransferrin) ELISA Kit | ELK3234 | Gentaur UK, US & Europe Distribution
Alternative Names: CD228; MAP97; MTF1; Antigen P97 (Melanoma Associated) ; Membrane-Bound Transferrin-Like Protein
Species: Human
Assay Type: Sandwich
Sensitivity: 12.9 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Dermatology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanotransferrin (MFI2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Melanotransferrin (MFI2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanotransferrin (MFI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanotransferrin (MFI2) in the samples is then determined by comparing the OD of the samples to the standard curve.