Human Mitochondrial fission 1 protein (FIS1) ELISA Kit | KTE62257

Human Mitochondrial fission 1 protein (FIS1) ELISA Kit | KTE62257 | Gentaur UK, US & Europe Distribution

Application: This Human Mitochondrial fission 1 protein (FIS1) ELISA Kit employs a two-site sandwich ELISA to quantitate FIS1 in samples. An antibody specific for FIS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFIS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FIS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FIS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Mitochondrial fission 1 protein microplate
• Human Mitochondrial fission 1 protein standard
• Human Mitochondrial fission 1 protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Mitochondrial fission 1 protein (FIS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human FIS1. No significant cross-reactivity or interference between Human FIS1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission. The deduced 152-amino acid protein contains a central leucine zipper, a coiled-coil region, and a C-terminal transmembrane domain. It also contains a region showing high interspecies homology and a putative tetratricopeptide repeat. Immunocytochemical analysis of HeLa cells detected endogenous FIS1 expression in mitochondria. Protease treatment indicated that FIS1 is expressed at the outer mitochondrial membrane. Transfection of a C-terminal truncated FIS1 resulted in cytoplasmic expression, indicating that the C terminus is required for mitochondrial localization.

Alternative Names: FIS1; CGI-135; TTC11; H_NH0132A01.6; tetratricopeptide repeat domain 11

Search name: FIS1; CGI-135; TTC11; H_NH0132A01.6; tetratricopeptide repeat domain 11

Tag: FIS1