Description
Human MPI (Mannose Phosphate Isomerase) ELISA Kit | ELK4329 | Gentaur UK, US & Europe Distribution
Alternative Names: PMI1; Mannose-6-Phosphate Isomerase; Phosphohexomutase; Phosphomannose isomerase
Species: Human
Assay Type: Sandwich
Sensitivity: 6.7 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Phosphate Isomerase (MPI) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mannose Phosphate Isomerase (MPI) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Phosphate Isomerase (MPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Phosphate Isomerase (MPI) in the samples is then determined by comparing the OD of the samples to the standard curve.