Description
Human MPP6 (Membrane Protein, Palmitoylated 6) ELISA Kit | ELK1906 | Gentaur UK, US & Europe Distribution
Alternative Names: PALS2; VAM1; P55T; MAGUK P55 Subfamily Member 6; Veli-associated MAGUK 1
Species: Human
Assay Type: Sandwich
Sensitivity: 4.7 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Lenght: 3.5h
Research Area: Signal transduction;Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Protein, Palmitoylated 6 (MPP6) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Membrane Protein, Palmitoylated 6 (MPP6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Membrane Protein, Palmitoylated 6 (MPP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Membrane Protein, Palmitoylated 6 (MPP6) in the samples is then determined by comparing the OD of the samples to the standard curve.