Human Multidrug resistance-associated protein 4 (ABCC4) ELISA Kit | AE23133HU

Human Multidrug resistance-associated protein 4 (ABCC4) ELISA Kit | AE23133HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: ABCC4

Alternative Name: RP11-74A12.1; EST170205; MOAT-B; MOATB; MRP4; ATP-binding cassette; sub-family C; member 4|bA464I2.1 (ATP-binding cassette; sub-family C (CFTR/MRP) ; member 4) |canalicular multispecific organic anion

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.115 ng/mL

Intra-Assay: ≤4.5%

Inter-Assay: ≤8.3%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate ABCC4 in samples. An antibody specific for ABCC4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: ABCC4, also known as MRP4, belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells. ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus. The insertion was attributed to 2 additional exons, designated 1a and 1b, that produced premature termination codons (PTCs) . A comparison of human, monkey, and rodent ABCC4 genes revealed that these same PTC-producing exons were also highly conserved in evolution. In vitro inhibition of protein synthesis (using puromycin or anisomycin) selectively reduced nonsense-mediated mRNA decay of PTC-containing ABCC4 transcripts in all cell lines tested.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .