Human Myosin-10 (MYH10) ELISA Kit | KTE62224

Human Myosin-10 (MYH10) ELISA Kit | KTE62224 | Gentaur UK, US & Europe Distribution

Application: This Human Myosin-10 (MYH10) ELISA Kit employs a two-site sandwich ELISA to quantitate MYH10 in samples. An antibody specific for MYH10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYH10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYH10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYH10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Myosin-10 microplate
• Human Myosin-10 standard
• Human Myosin-10 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Myosin-10 (MYH10) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYH10. No significant cross-reactivity or interference between Human MYH10 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Myh10 was abundantly expressed in mouse brain and testis. It was also expressed in heart, lung, liver, and kidney, but not in skeletal muscle or spleen.Actin-activated MgATPase activity was decreased in MYH10 with either an asn97-to-lysine substitution, which is homologous to the N93K mutation in MYH9 that causes May-Hegglin anomaly, or an arg709-to-cysteine substitution, which causes developmental defects in brain and heart when present in mouse Myh10. The ability of MYH10 heavy meromyosin to support the movement of actin filaments over an MYH10-coated surface was reduced in heavy meromyosin with the N97K mutation and eliminated with the R709C mutation. Kinetic analysis indicated that the R709C mutation resulted in extremely tight affinity between MYH10 heavy meromyosin and ADP, reducing the rate of ADP release.

Alternative Names: MYH10; MGC134913; MGC134914; NMMHCB; cellular myosin heavy chain; type B type B; myosin heavy chain; nonmuscle type B; myosin; heavy polypeptide 10; non-muscle; near to the ATP binding region; nonmuscle myosin

Search name: MYH10; MGC134913; MGC134914; NMMHCB; cellular myosin heavy chain; type B type B; myosin heavy chain; nonmuscle type B; myosin; heavy polypeptide 10; non-muscle; near to the ATP binding region; nonmuscle myosin

Tag: MYH10