Human Myotonin-protein kinase (DMPK) ELISA Kit | KTE62013

Human Myotonin-protein kinase (DMPK) ELISA Kit | KTE62013 | Gentaur UK, US & Europe Distribution

Application: This Human Myotonin-protein kinase (DMPK) ELISA Kit employs a two-site sandwich ELISA to quantitate DMPK in samples. An antibody specific for DMPK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMPK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMPK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMPK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Myotonin-protein kinase microplate
• Human Myotonin-protein kinase standard
• Human Myotonin-protein kinase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Myotonin-protein kinase (DMPK) ELISA Kit has high sensitivity and excellent specificity for detection of Human DMPK. No significant cross-reactivity or interference between Human DMPK and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Myotonic dystrophy protein kinase is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5, 000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Alternative Names: DMPK; DM; DM1; DM1PK; DMK; MDPK; MT-PK; DM protein kinase; myotonic dystrophy associated protein kinase; myotonic dystrophy protein kinase; myotonin protein kinase A; thymopoietin homolog

Search name: DMPK; DM; DM1; DM1PK; DMK; MDPK; MT-PK; DM protein kinase; myotonic dystrophy associated protein kinase; myotonic dystrophy protein kinase; myotonin protein kinase A; thymopoietin homolog

Tag: DMPK