Human NADH-ubiquinone oxidoreductase chain 5 (MT-ND5) ELISA Kit | KTE61486

Human NADH-ubiquinone oxidoreductase chain 5 (MT-ND5) ELISA Kit | KTE61486 | Gentaur UK, US & Europe Distribution

Application: This Human NADH-ubiquinone oxidoreductase chain 5 (MT-ND5) ELISA Kit employs a two-site sandwich ELISA to quantitate MT-ND5 in samples. An antibody specific for MT-ND5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT-ND5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT-ND5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT-ND5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human NADH-ubiquinone oxidoreductase chain 5 microplate
• Human NADH-ubiquinone oxidoreductase chain 5 standard
• Human NADH-ubiquinone oxidoreductase chain 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human NADH-ubiquinone oxidoreductase chain 5 (MT-ND5) ELISA Kit has high sensitivity and excellent specificity for detection of Human MT-ND5. No significant cross-reactivity or interference between Human MT-ND5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MT-ND5 (Mitochondrially Encoded NADH:Ubiquinone Oxidoreductase Core Subunit 5) is a Protein Coding gene. Diseases associated with MT-ND5 include Leber Optic Atrophy and Leigh Syndrome. Among its related pathways are Metabolism and Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.. GO annotations related to this gene include NADH dehydrogenase (ubiquinone) activity. Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity) .

Alternative Names: MT-ND5; MTND5; NADH dehydrogenase subunit 5

Search name: MT-ND5; MTND5; NADH dehydrogenase subunit 5

Tag: MT-ND5