Human Neuroendocrine convertase 1 (PCSK1) ELISA Kit | AE28364HU

Human Neuroendocrine convertase 1 (PCSK1) ELISA Kit | AE28364HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PCSK1

Alternative Name: BMIQ12; NEC1; PC1; PC3; SPC3; neuroendocrine convertase 1|prohormone convertase 1|prohormone convertase 3|proprotein convertase 1

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.101 ng/mL

Intra-Assay: ≤4.9%

Inter-Assay: ≤9.0%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PCSK1 in samples. An antibody specific for PCSK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Proprotein Convertase Subtilisin/Kexin Type 1 is aprotein belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity. This encoded protein is associated with carcinoid tumors. The use of alternate polyadenylation sites has been found for this gene. Multiple alternatively spliced transcript variants have been described for this gene but their full length nature is not known.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .