Human NGF (Nerve Growth Factor) CLIA Kit | E-CL-H0787

Human NGF (Nerve Growth Factor) CLIA Kit | E-CL-H0787 | Gentaur UK, US & Europe Distribution

Type: Sandwich

Assay Time: 3.5h

Sensitivity: 4.69pg/mL

Detection Range: 7.81~500pg/mL

UniProt ID: N/A

Target Name: NGF

Target Synonym: Beta-NGF, HSAN5, NGFB, NGF-B

Test Principale: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NGF. You can calculate the concentration of Human NGF in the samples by comparing the RLU value of the samples to the standard curve.

Specifivity: This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed.

Research Areas: Cancer, Neuroscience

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.