Description
Human NPSR (Neuropeptide S Receptor) ELISA Kit | ELK4254 | Gentaur UK, US & Europe Distribution
Alternative Names: ASRT2; GPR154; GPRA; NPSR1; PGR14; VRR1; G Protein-Coupled Receptor For Asthma Susceptibility; G Protein-Coupled Receptor 154; G-protein coupled receptor PGR14
Species: Human
Assay Type: Sandwich
Sensitivity: 0.108 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Neuro science
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropeptide S Receptor (NPSR) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuropeptide S Receptor (NPSR) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropeptide S Receptor (NPSR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropeptide S Receptor (NPSR) in the samples is then determined by comparing the OD of the samples to the standard curve.