855
Human NT-ProCNP (N-Terminal Pro-C-Type Natriuretic Peptide) ELISA Kit | ELK4727
- SKU:
- 855-ELK4727-GEN
Description
Human NT-ProCNP (N-Terminal Pro-C-Type Natriuretic Peptide) ELISA Kit | ELK4727 | Gentaur UK, US & Europe Distribution
Alternative Names: N-CNP
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 5.52 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Lenght: 2h
Research Area: Cardiovascular biology;Hormone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Terminal Pro-C-Type Natriuretic Peptide (NT-ProCNP) in the samples is then determined by comparing the OD of the samples to the standard curve.