Human NURR1 (Nuclear Receptor Related Protein 1) ELISA Kit | ELK7437

Human NURR1 (Nuclear Receptor Related Protein 1) ELISA Kit | ELK7437 | Gentaur UK, US & Europe Distribution

Alternative Names: NR4A2; RNR1; HZF-3; NOT; TINUR; Nuclear Receptor Subfamily 4, Group A, Member 2; Transcriptionally-inducible nuclear receptor; Immediate-early response protein NOT

Species: Human

Assay Type: Sandwich

Sensitivity: 0.118 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Related Protein 1 (NURR1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nuclear Receptor Related Protein 1 (NURR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Related Protein 1 (NURR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Receptor Related Protein 1 (NURR1) in the samples is then determined by comparing the OD of the samples to the standard curve.