Human One cut domain family member 2 (ONECUT2) ELISA Kit | KTE62362

Human One cut domain family member 2 (ONECUT2) ELISA Kit | KTE62362 | Gentaur UK, US & Europe Distribution

Application: This Human One cut domain family member 2 (ONECUT2) ELISA Kit employs a two-site sandwich ELISA to quantitate ONECUT2 in samples. An antibody specific for ONECUT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyONECUT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ONECUT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ONECUT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human One cut domain family member 2 microplate
• Human One cut domain family member 2 standard
• Human One cut domain family member 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human One cut domain family member 2 (ONECUT2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ONECUT2. No significant cross-reactivity or interference between Human ONECUT2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: HNF6a encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation. This CCDS representation uses the 5'-most in-frame start codon, which is conserved in other vertebrates, including mouse, opossum, pig and zebrafish. An alternative downstream start codon, which has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 20 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo.

Alternative Names: ONECUT2; MGC120377; MGC120378; OC-2; OC2; ONECUT-2 homeodomain transcription factor; one cut domain; family member 2; onecut 2

Search name: ONECUT2; MGC120377; MGC120378; OC-2; OC2; ONECUT-2 homeodomain transcription factor; one cut domain; family member 2; onecut 2

Tag: ONECUT2