Human Orexigenic neuropeptide QRFP (QRFP) ELISA Kit | KTE61002

Human Orexigenic neuropeptide QRFP (QRFP) ELISA Kit | KTE61002 | Gentaur UK, US & Europe Distribution

Application: This Human Orexigenic neuropeptide QRFP (QRFP) ELISA Kit employs a two-site sandwich ELISA to quantitate QRFP in samples. An antibody specific for QRFP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQRFP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QRFP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRFP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Orexigenic neuropeptide QRFP microplate
• Human Orexigenic neuropeptide QRFP standard
• Human Orexigenic neuropeptide QRFP detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Orexigenic neuropeptide QRFP (QRFP) ELISA Kit has high sensitivity and excellent specificity for detection of Human QRFP. No significant cross-reactivity or interference between Human QRFP and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: P518 functions as a high-affinity ligand of GPR103. Both GPR103 and P518 precursor mRNA exhibited highest expression in brain.The 43-amino acid QRFP peptide, a longer form of the P518 peptide is necessary to exhibit full agonistic activity with GPR103. Intravenous administration QRFP caused release of aldosterone, suggesting that QRFP and GPR103 regulate adrenal function.The deduced 126-amino acid protein contains a putative N-terminal 22-amino acid signal peptide and no transmembrane domain, suggesting that the protein or cleavage products can be secreted. The 26-amino acid P518 peptide sequence is located at the C terminus of the precursor protein. In peripheral tissues, P518 precursor mRNA was detected in prostate, testis, colon, thyroid, parathyroid, coronary artery, and bladder.

Alternative Names: QRFP; RP11-618A20.5; 26RFa; MGC119794; P518; P518 precursor protein; RF (Arg-Phe) amide family 26 amino acid peptide; RF (Arg-Phe) amide family 26 amino acid peptide (P518)

Search name: QRFP; RP11-618A20.5; 26RFa; MGC119794; P518; P518 precursor protein; RF (Arg-Phe) amide family 26 amino acid peptide; RF (Arg-Phe) amide family 26 amino acid peptide (P518)

Tag: QRFP