Human Pappalysin-2 (PAPPA2) ELISA Kit | KTE62361

Human Pappalysin-2 (PAPPA2) ELISA Kit | KTE62361 | Gentaur UK, US & Europe Distribution

Application: This Human Pappalysin-2 (PAPPA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PAPPA2 in samples. An antibody specific for PAPPA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPAPPA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAPPA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAPPA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Pappalysin-2 microplate
• Human Pappalysin-2 standard
• Human Pappalysin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Pappalysin-2 (PAPPA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PAPPA2. No significant cross-reactivity or interference between Human PAPPA2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Pappalysins comprise one of the five families of Metzincins (other families include ADAMs/ADAMTSs, MMPs, Astacins, and Serralysins) . Pappalysin-1 (PAPP-A) is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy. Reduced maternal serum PAPP-A is a major marker of Down syndrome in the first trimester of pregnancy. PAPP-A cleaves IGFBP-4 and -5 (insulin-like growth factor-binding protein-4 and 5) at a single site, resulting in the release of bioactive IGF. Pappalysin-2 (PAPP-A2; also called PAPP-E) shares 45% amino acid identity with PAPP-A and cleaves IGFBP-2.sialic acid binding Ig-like lectin 6 (Siglec-6), a potential leptin receptor, and pappalysin-2 (PAPP-A2), a protease that cleaves IGF-binding proteins

Alternative Names: PAPPA2; RP4-774I24.1; PAPP-A2; PAPPE; PLAC3; placenta-specific 3; pregnancy-associated plasma preproprotein-A2; pregnancy-associated plasma protein-E

Search name: PAPPA2; RP4-774I24.1; PAPP-A2; PAPPE; PLAC3; placenta-specific 3; pregnancy-associated plasma preproprotein-A2; pregnancy-associated plasma protein-E

Tag: PAPPA2