Human Patatin-like phospholipase domain-containing protein 4 (PNPLA4) ELISA Kit | KTE62355

Human Patatin-like phospholipase domain-containing protein 4 (PNPLA4) ELISA Kit | KTE62355 | Gentaur UK, US & Europe Distribution

Application: This Human Patatin-like phospholipase domain-containing protein 4 (PNPLA4) ELISA Kit employs a two-site sandwich ELISA to quantitate PNPLA4 in samples. An antibody specific for PNPLA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPNPLA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PNPLA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PNPLA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Patatin-like phospholipase domain-containing protein 4 microplate
• Human Patatin-like phospholipase domain-containing protein 4 standard
• Human Patatin-like phospholipase domain-containing protein 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Patatin-like phospholipase domain-containing protein 4 (PNPLA4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PNPLA4. No significant cross-reactivity or interference between Human PNPLA4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PNPLA4 encodes a member of the patatin-like family of phospholipases. The encoded enzyme has both triacylglycerol lipase and transacylase activities and may be involved in adipocyte triglyceride homeostasis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome Y.DNA sequencing of a GS2 cDNA clone demonstrated an open reading frame for a basic protein of 253 amino acid residues and an isoelectric point of 9.8. A polymorphic CT dinucleotide repeat was found in the 3-prime untranslated region. The GS2 gene was expressed in all human tissues examined, including heart, brain, placenta, lung, liver, muscle, kidney, pancreas, and spleen. Several GS2 transcripts, ranging in size from 1.1 to 5.8 kb, were found among different tissues, suggesting tissue-specific processing of the GS2 transcript.

Alternative Names: PNPLA4; DXS1283E; GS2; IPLA2-ETA; GS2

Search name: PNPLA4; DXS1283E; GS2; IPLA2-ETA; GS2

Tag: PNPLA4