Human PCI (Protein C Inhibitor) ELISA Kit | ELK3036

Human PCI (Protein C Inhibitor) ELISA Kit | ELK3036 | Gentaur UK, US & Europe Distribution

Alternative Names: SERPINA5; PAI3; PLANH3; PROCI; Serpin Peptidase Inhibitor Clade A 5; Plasma Serine Protease Inhibitor; Plasminogen Activator Inhibitor 3; Acrosomal serine protease inhibitor

Species: Human

Assay Type: Sandwich

Sensitivity: 13.7 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: plasma

Assay Lenght: 3.5h

Research Area: Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C Inhibitor (PCI) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein C Inhibitor (PCI) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C Inhibitor (PCI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein C Inhibitor (PCI) in the samples is then determined by comparing the OD of the samples to the standard curve.