855
Human PCSK2 (Proprotein Convertase Subtilisin/Kexin Type 2) ELISA Kit | ELK4709
- SKU:
- 855-ELK4709-GEN
Description
Human PCSK2 (Proprotein Convertase Subtilisin/Kexin Type 2) ELISA Kit | ELK4709 | Gentaur UK, US & Europe Distribution
Alternative Names: PC2; NEC2; SPC2; Prohormone Convertase 2; Neuroendocrine Convertase 2; KEX2-Like Endoprotease 2; Proprotein convertase 2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.112 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Enzyme & Kinase
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2) in the samples is then determined by comparing the OD of the samples to the standard curve.