Human PDCD1 (Programmed Cell Death Protein 1) ELISA Kit | ELK4353

Human PDCD1 (Programmed Cell Death Protein 1) ELISA Kit | ELK4353 | Gentaur UK, US & Europe Distribution

Alternative Names: CD279; PD1; SLEB2; HPD1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.063 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Apoptosis;Immune molecule;Endocrinology;Autoimmunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 (PDCD1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 (PDCD1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 1 (PDCD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Programmed Cell Death Protein 1 (PDCD1) in the samples is then determined by comparing the OD of the samples to the standard curve.