Human PDZRN3 (PDZ domain containing ring finger 3) ELISA Kit | ELK9138

Human PDZRN3 (PDZ domain containing ring finger 3) ELISA Kit | ELK9138 | Gentaur UK, US & Europe Distribution

Alternative Names: E3 ubiquitin-protein ligase PDZRN3; KIAA1095; Ligand of Numb protein X 3; likely ortholog of mouse semaF cytoplasmic domain associated protein 3; LNX3; PDZ domain containing ring finger 3; PDZ domain-containing RING finger protein 3; Pdzrn3; Protein SEMACAP3; PZRN3_HUMAN; SEMACAP3; Semaphorin cytoplasmic domain associated protein; Semaphorin cytoplasmic domain-associated protein 3; SEMCAP3

Species: Human

Assay Type: Sandwich

Sensitivity: 13.1 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.3-2000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Developmental Biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDZ domain containing ring finger 3 (PDZRN3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PDZ domain containing ring finger 3 (PDZRN3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDZ domain containing ring finger 3 (PDZRN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDZ domain containing ring finger 3 (PDZRN3) in the samples is then determined by comparing the OD of the samples to the standard curve.