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Human PEBP4 (Phosphatidylethanolamine Binding Protein 4) ELISA Kit | ELK5098
- SKU:
- 855-ELK5098-GEN
Description
Human PEBP4 (Phosphatidylethanolamine Binding Protein 4) ELISA Kit | ELK5098 | Gentaur UK, US & Europe Distribution
Alternative Names: CORK1; hPEBP4; Cousin-of-RKIP 1 Protein
Species: Human
Assay Type: Sandwich
Sensitivity: 0.119 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylethanolamine Binding Protein 4 (PEBP4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphatidylethanolamine Binding Protein 4 (PEBP4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylethanolamine Binding Protein 4 (PEBP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylethanolamine Binding Protein 4 (PEBP4) in the samples is then determined by comparing the OD of the samples to the standard curve.