Human Phosphatidylethanolamine N-methyltransferase (PEMT) ELISA Kit | AE28021HU

Human Phosphatidylethanolamine N-methyltransferase (PEMT) ELISA Kit | AE28021HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PEMT

Alternative Name: MGC2483; PEAMT; PEMPT; PEMT2; PNMT; OTTHUMP00000065537

Application: ELISA

Range: 25-1600 pg/mL

Sensitivity: 6.25 pg/mL

Intra-Assay: ≤5.5%

Inter-Assay: ≤10.3%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PEMT in samples. An antibody specific for PEMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPEMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEMT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEMT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Phosphatidylethanolamine N-methyltransferase (abbreviated PEMT) is a transferase enzyme (EC 2.1.1.17) which converts phosphatidylethanolamine (PE) to phosphatidylcholine (PC) in the liver. In humans it is encoded by the PEMT gene within the Smith-Magenis syndrome region on chromosome 17.While the CDP-choline pathway, in which choline obtained either by dietary consumption or by metabolism of choline-containing lipids is converted to PC, accounts for approximately 70% of PC biosynthesis in the liver, the PEMT pathway has been shown to have played a critical evolutionary role in providing PC during times of starvation. Furthermore, PC made via PEMT plays a wide range of physiological roles, utilized in choline synthesis, hepatocyte membrane structure, bile secretion, and very-low-density lipoprotein (VLDL) secretion.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .