Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) ELISA Kit | KTE62359

Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) ELISA Kit | KTE62359 | Gentaur UK, US & Europe Distribution

Application: This Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3R4 in samples. An antibody specific for PIK3R4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3R4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3R4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3R4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Phosphoinositide 3-kinase regulatory subunit 4 microplate
• Human Phosphoinositide 3-kinase regulatory subunit 4 standard
• Human Phosphoinositide 3-kinase regulatory subunit 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Phosphoinositide 3-kinase regulatory subunit 4 (PIK3R4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIK3R4. No significant cross-reactivity or interference between Human PIK3R4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: In yeast, the vps15 gene product is a 150-kD serine/threonine kinase that forms a complex with a phosphatidylinositol 3-kinase, vps34 (PIK3C3) ; in human this complex is involved in protein trafficking. By screening a cDNA library, Panaretou et al. (1997) cloned the homolog of the yeast vps15 gene, which they termed p150. The p150 cDNA encodes a predicted polypeptide of 1, 358 amino acids. The amino acid sequence is 29.6% identical to that of yeast vps15 protein. Northern blot analysis revealed that the p150 gene is ubiquitously expressed as a 5.5-kb transcript. Panaretou et al. (1997) found that the p150 gene interacts with PIK3C3 in vivo.

Alternative Names: PIK3R4; MGC102700; VPS15; p150; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; phosphatidylinositol 3-kinase-associated p150; phosphoinositide 3-kinase adaptor protein; phosphoinositide-3-kinase; reg

Search name: PIK3R4; MGC102700; VPS15; p150; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; phosphatidylinositol 3-kinase-associated p150; phosphoinositide 3-kinase adaptor protein; phosphoinositide-3-kinase; reg

Tag: PIK3R4