Human Phospholipase D3 (PLD3) ELISA Kit | AE27068HU

Human Phospholipase D3 (PLD3) ELISA Kit | AE27068HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PLD3

Alternative Name: HU-K4; HUK4; HindIII K4L|OTTHUMP00000202292|OTTHUMP00000202324|OTTHUMP00000202326|choline phosphatase 3|phosphatidylcholine-hydrolyzing phospholipase D3|phospholipase D3

Application: ELISA

Range: 78.1-5000 pg/mL

Sensitivity: 40 pg/mL

Intra-Assay: ≤5.8%

Inter-Assay: ≤7.5%

Recovery: 0, 93

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PLD3 in samples. An antibody specific for PLD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Hu-K4 is a human protein homologous to the K4L protein of vaccinia virus.Hu-K4 was assigned to the family of Phospholipase D proteins although so far no catalytic activity has been shown. The Hu-K4 mRNA is found in many human organs with highest expression levels in the central nervous system. As a consequence the protein is 53 amino acids larger than originally predicted, now harbouring a putative transmembrane domain. The exon/intron structure of the Hu-K4 gene reveals extensive alternative splicing in the 5' untranslated region. Due to the absence of G/C-rich regions and upstream ATG codons, the mRNA isoform in brain may be translated with higher efficacy leading to a high Hu-K4 protein concentration in this tissue. Hu-K4 is a membrane-bound protein colocalizing with protein disulfide isomerase, a marker of the endoplasmic reticulum.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .