Human Phosphotidylinositol phosphatase PTPRQ (PTPRQ) ELISA Kit | AE25047HU

Human Phosphotidylinositol phosphatase PTPRQ (PTPRQ) ELISA Kit | AE25047HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PTPRQ

Alternative Name: PTPGMC1; protein-tyrosine phosphatase; receptor-type; expressed by glomerular mesangial cells

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.058 ng/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤8.6%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PTPRQ in samples. An antibody specific for PTPRQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPRQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: PTPRQ belongs to the type III receptor-like protein-tyrosine phosphatase (PTPase) family. PTPRQ has low activity against phosphotyrosine, but is active against phosphatidylinositol phosphates that are involved in regulation of survival, proliferation, and subcellular architecture. The genomic region containing nt 391..910 of this sequence are inverted in the human reference genome (build 37) . This portion of the RefSeq is inferred based on alignments of rat and mouse transcripts, and is supported by the human Celera assembly. In developing kidney, it localizes to the basal membrane of podocytes, beginning when podocyte progenitors can first be identified in the embryonic kidney (at protein level) . Expressed in lung and kidney.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .