Human Phosphotidylinositol phosphatase PTPRQ (PTPRQ) ELISA Kit | KTE61045

Human Phosphotidylinositol phosphatase PTPRQ (PTPRQ) ELISA Kit | KTE61045 | Gentaur UK, US & Europe Distribution

Application: This Human Phosphotidylinositol phosphatase PTPRQ (PTPRQ) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPRQ in samples. An antibody specific for PTPRQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPRQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Phosphotidylinositol phosphatase PTPRQ microplate
• Human Phosphotidylinositol phosphatase PTPRQ standard
• Human Phosphotidylinositol phosphatase PTPRQ detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Phosphotidylinositol phosphatase PTPRQ (PTPRQ) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTPRQ. No significant cross-reactivity or interference between Human PTPRQ and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PTPRQ belongs to the type III receptor-like protein-tyrosine phosphatase (PTPase) family. PTPRQ has low activity against phosphotyrosine, but is active against phosphatidylinositol phosphates that are involved in regulation of survival, proliferation, and subcellular architecture. The genomic region containing nt 391..910 of this sequence are inverted in the human reference genome (build 37) . This portion of the RefSeq is inferred based on alignments of rat and mouse transcripts, and is supported by the human Celera assembly. In developing kidney, it localizes to the basal membrane of podocytes, beginning when podocyte progenitors can first be identified in the embryonic kidney (at protein level) . Expressed in lung and kidney.

Alternative Names: PTPRQ; PTPGMC1; protein-tyrosine phosphatase; receptor-type; expressed by glomerular mesangial cells

Search name: PTPRQ; PTPGMC1; protein-tyrosine phosphatase; receptor-type; expressed by glomerular mesangial cells

Tag: PTPRQ