Description
Human PIIINP (Procollagen III N-Terminal Propeptide) ELISA Kit | ELK5418 | Gentaur UK, US & Europe Distribution
Alternative Names: P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 2.13 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Lenght: 2h
Research Area: Metabolic pathway;Hepatology;Bone metabolism
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Procollagen III N-Terminal Propeptide (PIIINP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen III N-Terminal Propeptide (PIIINP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen III N-Terminal Propeptide (PIIINP) in the samples is then determined by comparing the OD of the samples to the standard curve.