Human Plastin-2 (LCP1) ELISA Kit | KTE61845

Human Plastin-2 (LCP1) ELISA Kit | KTE61845 | Gentaur UK, US & Europe Distribution

Application: This Human Plastin-2 (LCP1) ELISA Kit employs a two-site sandwich ELISA to quantitate LCP1 in samples. An antibody specific for LCP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Plastin-2 microplate
• Human Plastin-2 standard
• Human Plastin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Plastin-2 (LCP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LCP1. No significant cross-reactivity or interference between Human LCP1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.) . L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.

Alternative Names: LCP1; CP64; DKFZp781A23186; FLJ25423; FLJ26114; FLJ39956; L-PLASTIN; LC64P; LPL; PLS2; L-plastin; L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P) ; Lymphocyte cytosolic protein-1 (plasmin) ; OTTHUMP

Search name: LCP1; CP64; DKFZp781A23186; FLJ25423; FLJ26114; FLJ39956; L-PLASTIN; LC64P; LPL; PLS2; L-plastin; L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P) ; Lymphocyte cytosolic protein-1 (plasmin) ; OTTHUMP

Tag: LCP1