Human PLCg1 (Phospholipase C Gamma 1) ELISA Kit | ELK2093

Human PLCg1 (Phospholipase C Gamma 1) ELISA Kit | ELK2093 | Gentaur UK, US & Europe Distribution

Alternative Names: NCKAP3; PLC-G1; PLC-II; PLC1; PLC148; PLCgamma1; 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1; Phosphoinositide phospholipase C-gamma-1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase C Gamma 1 (PLCg1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phospholipase C Gamma 1 (PLCg1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase C Gamma 1 (PLCg1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase C Gamma 1 (PLCg1) in the samples is then determined by comparing the OD of the samples to the standard curve.