855
Human PLCg2 (Phospholipase C Gamma 2, Phosphatidylinositol Specific) ELISA Kit | ELK3787
- SKU:
- 855-ELK3787-GEN
Description
Human PLCg2 (Phospholipase C Gamma 2, Phosphatidylinositol Specific) ELISA Kit | ELK3787 | Gentaur UK, US & Europe Distribution
Alternative Names: PLC-IV; Phosphoinositide phospholipase C-gamma-2; 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-2
Species: Human
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Metabolic pathway
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase C Gamma 2, Phosphatidylinositol Specific (PLCg2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phospholipase C Gamma 2, Phosphatidylinositol Specific (PLCg2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase C Gamma 2, Phosphatidylinositol Specific (PLCg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase C Gamma 2, Phosphatidylinositol Specific (PLCg2) in the samples is then determined by comparing the OD of the samples to the standard curve.