Human Plexin-A2 (PLXNA2) ELISA Kit | AE26898HU

Human Plexin-A2 (PLXNA2) ELISA Kit | AE26898HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PLXNA2

Alternative Name: RP11-328D5.3; FLJ11751; FLJ30634; KIAA0463; OCT; PLXN2; plexin 2|plexin-A2|semaphorin receptor OCT|transmembrane protein OCT

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.078 ng/mL

Intra-Assay: ≤4.3%

Inter-Assay: ≤7.5%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PLXNA2 in samples. An antibody specific for PLXNA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLXNA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Plexin-A2 is a protein that in humans is coded by the PLXNA2 gene. This gene encodes a member of the plexin-A family of semaphorin co-receptors. Semaphorins are a large family of secreted or membrane-bound proteins that mediate repulsive effects on axon pathfinding during nervous system development. A subset of semaphorins are recognized by plexin-A/neuropilin transmembrane receptor complexes, triggering a cellular signal transduction cascade that leads to axon repulsion. This plexin-A family member is thought to transduce signals from semaphorin-3A and -3C. In some studies, the PLXNA2 gene is associated with schizophrenia and anxiety.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .