Human PRKR-interacting protein 1 (PRKRIP1) ELISA Kit | AE60043HU

Human PRKR-interacting protein 1 (PRKRIP1) ELISA Kit | AE60043HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PRKRIP1

Alternative Name: C114; FLJ13902; FLJ40957; likely ortholog of mouse C114 dsRNA-binding protein

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤4.6%

Inter-Assay: ≤8.3%

Recovery: 0, 99

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PRKRIP1 in samples. An antibody specific for PRKRIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKRIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKRIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKRIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The C114 cDNA contains an open reading frame of 187 amino acids with a predicted mass of 21 kDa. Using green fluorescent protein (GFP) -C114 fusion plasmids, amino acids 126-131 are shown to be essential for the nuclear localization of C114. An arginine-rich region (amino acids 98-143) spanning the nuclear localization signals (amino acids 126-131) exhibits a double-stranded RNA (dsRNA) binding activity. Competition experiments with different RNA homopolymers demonstrate that C114 preferentially binds to poly (I.C) . C114 binds to the dsRNA-activated protein kinase, protein kinase R (PKR), via dsRNA-binding domains of PKR and the N-terminal region of the C114 protein. In vitro kinase assays indicate that C114 inhibits PKR activation via a dsRNA-independent mechanism.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .